Tissues were washed overnight, immersed in an unbuffered solution of 1% osmium tetroxide for 1 hour, washed briefly, dehydrated in graded ethanols, cleared in xylene, and embedded in Araldite.
Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. The limited availability of therapeutic ...
Finally, the slides were dehydrated and mounted in xylene-based mounting media. Images were captured by Nikon Eclipse Ni microscope (Minato City, Tokyo, Japan) using ×20 magnification. Single-cell ...
PK-6100) was used to develop the staining. The sections were then mounted on gelatine-coated slides and dried overnight. Sections were dehydrated for 1 min in 90% and then 100% ethanol, incubated for ...
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