Approximately 25 ng (1–6 μl) of each product were combined in a final volume of 20 μl and ligated for 16 h at 4°C in 1× T4 ligase buffer with 3 Weiss U of T4 DNA ligase (NEB). Hydrolysis ...
DNA double-strand breaks (DSBs ... Through its E-rich motif, VGLL3 associates with KLHL15, an E3 ubiquitin ligase adapter, to ...
Figure 2: Ligation of circularizable probes by Ampligase, a thermostable DNA ligase. To facilitate allele discrimination in genomic DNA, we designed a second oligonucleotide primer to bind ...
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