For permeabilization, the cells were cultured for 24 h in the presence of Brefeldin A to block protein secretion and then fixed and permeabilized using 2% formaldehyde and 0.1% saponin before ...

2b) and HELA cells (not shown) were experimentally mimicked by permeabilizing cells with saponin and varying the amount of extracellular ATP. Already within a few minutes after the addition of ...

Researchers identify the crucial protein for controlling the biosynthesis of steroidal glycoalkaloids and saponins in plants of the genus Solanum and for the first time demonstrate the ecological ...