These soda lakes each contained ~1 M Na + and ~1 mM Mg 2+ at pH 10. The authors found that spontaneous extension of short RNA primers occurred in lake water at a rate comparable to the rates in ...

The ROC method entails using PCR primers that contain regions of RNA sequence that cannot be copied by certain thermostable DNA polymerases. Using such a chimeric primer in PCR would yield a ...

In addition to the template, DNA polymerases need a pre-existing DNA or RNA chain end (a primer) onto which to add each nucleotide. For this reason, the lagging strand polymerase requires the ...