The ROC method entails using PCR primers that contain regions of RNA sequence that cannot be copied by certain thermostable DNA polymerases. Using such a chimeric primer in PCR would yield a ...

These include difficulty in purifying extremely small amounts of total RNA, a lack of sensitivity, the need to collect samples often because of the very limited amount of RNA available and the ...

These soda lakes each contained ~1 M Na + and ~1 mM Mg 2+ at pH 10. The authors found that spontaneous extension of short RNA primers occurred in lake water at a rate comparable to the rates in ...