Continue collecting FFE fractions until there is no trace of bromophenol blue left in the IEF separation chamber. Store the fractions at 4 °C for subsequent RP-HPLC or SDS-PAGE or at −20 °C ...

This valuable study reveals extensive binding of eukaryotic translation initiation factor 3 (eIF3) to the 3' untranslated regions (UTRs) of efficiently translated mRNAs in human pluripotent stem ...