Furthermore, it permits parallel interrogation of multiple antigens. Plating hybridomas into methylcellulose-based semisolid medium was first described 25 years ago 1. Building on this method ...

Figure 3. Confirmation of successful amplification of antibody genes from mouse hybridomas. 克隆扩增产物至pUC118后测序。确认序列包含单克隆抗体H链和L链N端氨基酸序列对应的ORF。 我们对其他六种杂交瘤细胞系的抗体可变区进行同样的基因序列分析。获得了所有抗体亚类的PCR产物。

Polyclonals are prone to batch-to-batch variability when different animals are re-immunized, while the expression of monoclonals in hybridomas can vary over time, reducing specificity 3.

Ovarian cancer is a relatively manageable malignancy when diagnosed at an early stage, but late-stage detection almost always translates into a poor prognosis. Researchers have been vigorously ...