Fixed cells should be washed and suspended in a buffer that contains protein. (DPBS + 5% FBS) for longer term storage. They can be left in the fixative for up to two days. Store the fixed cells at 4°C ...
In general, mouse tissues should be fixed in formalin for 24 to 48 hours, but can be left much longer. Our standard fixative for rodent tissues is 75% ethanol-10% formalin. The ethanol speeds fixation ...
The nuclei are then treated with a chemical fixative, dropped on a glass slide, and treated with various stains that reveal structural features of the chromosomes. Banding Patterns Reveal the ...
Transcription activation of genes by estrogens is driven by enhancers, which are often located within the same Topologically Associating Domain (TAD) as non-targeted promoters. We investigated how ...