Heparin - Wikipedia
网页Heparin, also known as unfractionated heparin (UFH), is a medication and naturally occurring glycosaminoglycan. [3][4] Heparin is a blood anticoagulant that increases the activity of antithrombin. [5] It is used in the treatment of heart attacks and unstable angina. [3]
Isolation, Purification, and Characterization of Heparinase from
网页2019年4月24日 · The total heparinase production was higher for the intracellular enzyme (41.04 U/L) compared to the extracellular enzyme (34.02 U/L). A major amount of heparin was utilized in the stationary phase...
Identification and characterization of a novel heparinase ... - Nature
网页2023年11月17日 · Heparin (HP) and heparan sulfate (HS) are multifunctional polysaccharides widely used in clinical therapy. Heparinases (Hepases) are enzymes that specifically catalyse HP and HS degradation,...
Heparinase II from Flavobacterium heparinum - Journal of …
网页The three heparinases derived from Flavobacterium heparinum are powerful tools for studying heparin-like glycosaminoglycans in major biological processes, including angiogenesis and development. Heparinase II is unique among the three enzymes because it is able to catalytically cleave both heparin and heparan sulfate-like regions of heparin ...
Heparinase I from Flavobacterium heparinum - Journal of …
网页In this study we have identified the primary heparin binding site of heparinase I (EC 4.2.2.7). Chemical and proteolytic digests of heparinase I were used in direct binding and competition assays, to map the regions of heparinase I that interact specifically with heparin.
Heparinase I from Flavobacterium heparinum - Journal of …
网页Three enzymes that degrade HLGAGs (heparin and heparan sulfate), viz. heparinases I, II, and III from Flavobacterium heparinum, recognize unique sequences of sulfation and uronic acid epimerization in HLGAGs with a high degree of specificity (3-5).
Cloning, Expression, and Characterization of a Highly Stable …
网页2023年4月3日 · Heparinase I was first discovered in Flavobacterium heparinum , while it has been found to be produced by other microorganisms including several Bacteroides [17,18,19,20]. Production of native heparinase with the original bacteria is hampered by several obstacles, including variability in production [ 20 ], costly heparin-based inducers [ …
Purification and characterization of heparinase from …
网页1985年2月10日 · Heparinase (EC 4.2.2.7) isolated from Flavobacterium heparinum was purified to homogeneity by a combination of hydroxylapatite chromatography, repeated gel filtration chromatography, and chromatofocusing.
Heparinase production by Flavobacterium heparinum. - PMC
网页Heparinase production by Flavobacterium heparinum in complex protein digest medium, with heparin employed as the inducer, has been studied and improved. The maximum productivity of heparinase has been increased 156-fold over that achieved by previously published methods to 375 U/liter per h in the complex medium.
Heparin/Heparan Sulfate 6- O -Sulfatase from Flavobacterium heparinum
网页2009年12月11日 · In this study, we report the molecular cloning and recombinant expression in Escherichia coli of the F. heparinum sulfohydrolase, namely the glucosamine 6-O-sulfatase, and in the accompanying manuscript we report detailed studies on the N-sulfamidase as well as a model for how the 2-O- and 6-O-sulfatases, and N-sulfamidase work in concert. In ...