Sample preparation for SEM of plant surfaces - ScienceDirect
网页2010年1月1日 · Scanning electron microscopy (SEM) is an ideal technique for examining plant surfaces at high resolution. Plant tissues must be preserved by dehydration for observation in an electron microscope because the coating system and the microscopes operate under high vacuum and most specimens cannot withstand water removal by the vacuum system without ...
Cell surface and cell outline imaging in plant tissues using the ...
网页2013年10月17日 · Scanning electron microscopy (SEM) has been used for high-resolution imaging of plant cell surfaces for many decades. Most SEM imaging employs the secondary electron detector under high vacuum to provide pseudo-3D images of plant organs and especially of surface structures such as trichomes and stomatal guard cells; these samples generally have ...
An Overview of Cryo-Scanning Electron Microscopy Techniques for Plant …
网页2022年4月20日 · CryoSEM characterisation of phase separation within plant cells is a useful tool for understanding cell function. This has been applied to, for example, anthocyanic vacuolar inclusions and oil droplets within the cell.
Electron microscopy for imaging organelles in plants and algae | Plant …
网页2021年9月21日 · Recent developments in both instrumentation and image analysis algorithms have allowed three-dimensional electron microscopy (3D-EM) to increase automated image collections through large tissue volumes using serial block-face scanning EM (SEM) and to achieve near-atomic resolution of macromolecular complexes using cryo-electron tomography (cryo-...
Volumetric 3D reconstruction of plant leaf cells using SEM
网页2022年5月6日 · The 3D reconstruction of cells through serial sectioning transmission electron microscopy (ssTEM) and focused ion beam scanning electron microscopy (FIB-SEM) are powerful alternatives but not widely used in plant sciences.
Fast and cost-effective preparation of plant cells for scanning ...
网页2020年11月15日 · A fast and inexpensive preparation protocol of plant cell for viewing by SEM was provided for the first time. • The plant cells can be chemically stabilized with glutaraldehyde (5%) for 1 h. • This protocol can be interestingly used by plant cell biologists.
A Scanning Electron Microscopy Technique for Viewing Plant…
网页2019年5月15日 · Here we present a method in which we section paraffin-embedded samples to first find a region of interest in a light microscope and subsequently use scanning electron microscopy (SEM) to generate high-resolution images of plant-pathogen interactions in specific plant cell types.
Different Imaging Techniques for the 2 and 3D Characterization of Plant …
网页2023年7月22日 · This study provides an overview of SEM and TEM techniques for the rapid evaluation of 2D ultrastructural changes in plants. Additionally, methods for the 3D reconstruction and volume extraction of plant cells based on serial section TEM (ssTEM), focused ion beam SEM (FIB-SEM) are demonstrated.
Fast and cost-effective preparation of plant cells for scanning ...
网页2020年11月15日 · In the present study, a fast and cost-effective protocol was first performed for chemical fixation and preparation of the plant cells for imaging by SEM. Taxus baccata and Zhumeria majdae cells were chemically fixed using glutaraldehyde and then successfully dried with different percentages of ethanol including 70, 80, 90, and 100%.
A label-free, fast and high-specificity technique for plant cell …
网页2021年3月19日 · Plant cell walls are a renewable biomaterial composed of lignin and various polysaccharides in a complex three-dimensional matrix. The chemical composition of plant cell walls varies not only between tissues and cell types but also along developmental gradients and even within a single cell wall [1, 2, 3].