Current view and perspectives in viroid replication - PMC
网页PSTVd is the type species of Pospiviroidae. In contrast, members of Avsunviroidae adopt a highly branched structure at one end of their RNA genome, replicate via the symmetric rolling-circle model in chloroplasts, and possess hammerhead ribozymes. Avocado sunblotch viroid (ASBVd) is the type species of Avsunviroidae.
Modes of Viroid Transmission - PMC - National Center for …
网页2022年2月18日 · In 1971, Diener demonstrated that the causal agent of potato spindle disease, first described in the early 1920’s, is a small, highly structured, covalently closed circular RNA known as potato-spindle-tuber viroid (PSTVd) [1,2].
Potato spindle tuber viroid - PMC - National Center for …
网页Here we review recent literature data concerning the genetics of Potato spindle tuber viroid (PSTVd) and the mechanisms related to its pathological effect on the host plants. PSTVd can be transmitted vertically through microspores and macrospores, but not with pollen from another infected plant.
Transcription of potato spindle tuber viroid by RNA polymerase II ...
网页2001年11月15日 · Pospiviroidae, with their main representative potato spindle tuber viroid (PSTVd), are replicated via a rolling circle mechanism by the host-encoded DNA-dependent RNA polymerase II (pol II). In the first step, the (+)-strand circular viroid is transcribed into a (–)-strand oligomer intermediate.
A viroid-derived system to produce large amounts of ... - Nature
网页2018年1月30日 · Most viroids, like Potato spindle tuber viroid (PSTVd), contain a central conserved region (CCR), approximately in the middle of the folded molecule, and replicate in the nucleus of infected...
Cellular roadmaps of viroid infection: Trends in Microbiology - Cell …
网页2023年6月20日 · Avocado sunblotch viroid (ASBVd), the type species of the Avsunviroidae family, was reported to replicate in Saccharomyces cerevisiae and a cyanobacterium that lack chloroplasts. Future investigations are needed to help interpret these observations.
Processing of Potato Spindle Tuber Viroid RNAs in Yeast, a ...
网页2017年11月30日 · Potato spindle tuber viroid (PSTVd) is a circular, single-stranded, noncoding RNA plant pathogen that is a useful model for studying the processing of noncoding RNA in eukaryotes. Infective PSTVd circles are replicated via an asymmetric rolling circle mechanism to form linear multimeric RNAs.
Molecular biology of viroid–host interactions and disease control ...
网页2014年11月1日 · PSTVd infection of tomato plants has been shown to selectively alter the phosphorylation state of the host-encoded protein p68 that led to activation of its dsRNA-dependent protein kinase activity [124]. Immunological assays revealed that this phosphoprotein was related to a dsRNA-dependent protein kinase from virus-infected, interferon-treated ...
Characterization of nuclear import of potato spindle tuber viroid …
网页2002年1月5日 · We have characterized nuclear import of infectious, fluorescein-labeled potato spindle tuber viroid (F-PSTVd) in permeabilized tobacco BY2 cells. Import was observed for F-PSTVd but not for mRNA fragments of the same size or two viroids believed to replicate in the chloroplasts.
Transcription of potato spindle tuber viroid by RNA polymerase II ...
网页2006年4月10日 · Viroids are single-stranded, circular RNAs of 250 to 400 bases, that replicate autonomously in their host plants but do not code for a protein. Viroids of the family Pospiviroidae, of which potato spindle tuber viroid (PSTVd) is the type strain, are replicated by the host's DNA-dependent RNA polymerase II in the nucleus.