Bioluminescence Resonance Energy Transfer (BRET) Imaging in Plant ...
网页Here, we report methods for BRET imaging or (I) localization of COP1 dimerization in plant cells and tissues and subcellular distributions of interactions of the CCAAT/Enhancer Binding Protein α (C/EBPα) in single mammalian cells. We also discuss methods for the correction of BRET images for tissues that absorb light of different spectra.
Bioluminescence resonance energy transfer–based imaging of …
网页2019年3月25日 · RET is a natural phenomenon occurring between two photoactive molecules 1. It corresponds to the transfer of energy from a donor to an acceptor molecule through a non-radiative resonance process...
Bioluminescence resonance energy transfer (BRET) for the real …
网页2006年6月27日 · What is BRET? BRET is a naturally occurring phenomenon used in the laboratory for real-time monitoring of protein-protein interactions in live cells, cell extracts or purified preparations 1,...
Bioluminescence Resonance Energy Transfer (BRET) Imaging in Plant ...
网页2010年1月1日 · Here, we report methods for BRET imaging of (1) localization of COP1 dimerization in plant cells and tissues and (2) subcellular distributions of interactions of the CCAAT/Enhancer Binding Protein α (C/EBPα) in single mammalian cells. We also discuss methods for the correction of BRET images for tissues that absorb light of different spectra.
Illuminating insights into protein-protein interactions using
网页2006年2月17日 · What is BRET? BRET is an intrinsic process that can be observed in sea creatures such as the jellyfish Aequorea victoria and sea pansy Renilla reniformis. It involves nonradiative...
In Vivo Detection of Protein-Protein Interaction in Plant Cells Using BRET
网页The emerging technique of bioluminescence resonance energy transfer (BRET) allows us to detect protein interactions in live cells and in real time, thus providing a new window into cellular signal transduction processes. We present experimental protocols for...
网页Introduction. Protein interactions are important for coordinating cellular signaling events as well as metabolic functions in any cell. Numerous techniques have been devel-oped to detect and study protein–protein interactions either in vitro or in vivo.
[PDF] Imaging protein interactions with bioluminescence …
网页2007年6月12日 · A new generation electron bombardment-charge-coupled device camera coupled to an image splitter is demonstrated that BRET can be used to image protein interactions in plant and animal cells and in tissues; even subcellular imaging is possible. Expand. View on NAS. europepmc.org. Save to Library. Create Alert. …
Imaging protein interactions with bioluminescence resonance …
网页2007年6月12日 · Using a new generation electron bombardment-charge-coupled device camera coupled to an image splitter, we demonstrate that BRET can be used to image protein interactions in plant and animal cells and in …
A suite of tools and application notes for in vivo protein …
网页Bioluminescence resonance energy transfer (BRET) is a natural biophysical phenomenon that underlies an emerging technique to monitor protein-protein interactions in living cells in real time. Here, we present a series of technical advances to enhance the utility of the BRET assay in plants.